Mechanism of membrane perforation in rotavirus cell entry

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Cell entry of nonenveloped animal viruses requires translocation of a macromolecular assembly across a cellular membrane. Double-stranded RNA viruses introduce into the target cell an inner capsid particle that does not uncoat further. Instead, it extrudes capped viral mRNA by virtue of polymerase and capping activities within it. As described here, we used cryogenic electron tomography to visualize the full course of rhesus rotavirus entry, from cell attachment and virion uptake to release of the subviral particle.

Pumping up the volume

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The time and cost of annotating ground-truth images and network training are major challenges to utilizing machine learning to automate the mining of volume electron microscopy data. In this work we present a less computationally intense pipeline to train a convolutional neural network aimed at rapid automated detection of intracellular structures in volume electron microscopy using a limited number of loosely annotated images.  Find out more...